This study demonstrates the sustainable production of short- and medium-chain-length polyhydroxyalkanoates (PHA) using Pseudomonas frigusceleri MPC6 strain from crude glycerol as a renewable feedstock. Batch and fed-batch bioreactor experiments were conducted to optimize PHA accumulation. The results show that P. frigusceleri MPC6 can efficiently convert crude glycerol into high-molecular-weight PHAs with tailored monomeric compositions. Fed-batch cultivation with optimized feeding strategies achieved high PHA yields and productivities, making this bioprocess suitable for sustainable industrial-scale production of value-added biopolymers from waste glycerol streams.